tx tbs Search Results


lysis buffer 1  (Thermo Fisher)
96
Thermo Fisher lysis buffer 1
Lysis Buffer 1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/lysis buffer 1/product/Thermo Fisher
Average 96 stars, based on 1 article reviews
lysis buffer 1 - by Bioz Stars, 2026-02
96/100 stars
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p21  (Santa Cruz Biotechnology)
96
Santa Cruz Biotechnology p21
Figure 5. (A) SIRT1, FoxO3a, p53 and <t>p21</t> protein levels in lung tissue samples, analyzed by western blot, with β‑actin as a loading control. (B) SIRT1 deacetylase activity in lung samples. All analyses were performed in triplicate. Values are presented as the mean + standard deviation. *P<0.05 vs. control group. SIRT1, sirtuin 1; FoxO3a, forkhead box O 3a; COPD, chronic obstructive pulmonary disease.
P21, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/p21/product/Santa Cruz Biotechnology
Average 96 stars, based on 1 article reviews
p21 - by Bioz Stars, 2026-02
96/100 stars
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tbs tx  (Thermo Fisher)
99
Thermo Fisher tbs tx
Figure 5. (A) SIRT1, FoxO3a, p53 and <t>p21</t> protein levels in lung tissue samples, analyzed by western blot, with β‑actin as a loading control. (B) SIRT1 deacetylase activity in lung samples. All analyses were performed in triplicate. Values are presented as the mean + standard deviation. *P<0.05 vs. control group. SIRT1, sirtuin 1; FoxO3a, forkhead box O 3a; COPD, chronic obstructive pulmonary disease.
Tbs Tx, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/tbs tx/product/Thermo Fisher
Average 99 stars, based on 1 article reviews
tbs tx - by Bioz Stars, 2026-02
99/100 stars
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triton x-100  (Merck & Co)
90
Merck & Co triton x-100
Figure 5. (A) SIRT1, FoxO3a, p53 and <t>p21</t> protein levels in lung tissue samples, analyzed by western blot, with β‑actin as a loading control. (B) SIRT1 deacetylase activity in lung samples. All analyses were performed in triplicate. Values are presented as the mean + standard deviation. *P<0.05 vs. control group. SIRT1, sirtuin 1; FoxO3a, forkhead box O 3a; COPD, chronic obstructive pulmonary disease.
Triton X 100, supplied by Merck & Co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/triton x-100/product/Merck & Co
Average 90 stars, based on 1 article reviews
triton x-100 - by Bioz Stars, 2026-02
90/100 stars
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slides in vectashield  (Vector Laboratories)
86
Vector Laboratories slides in vectashield
Figure 5. (A) SIRT1, FoxO3a, p53 and <t>p21</t> protein levels in lung tissue samples, analyzed by western blot, with β‑actin as a loading control. (B) SIRT1 deacetylase activity in lung samples. All analyses were performed in triplicate. Values are presented as the mean + standard deviation. *P<0.05 vs. control group. SIRT1, sirtuin 1; FoxO3a, forkhead box O 3a; COPD, chronic obstructive pulmonary disease.
Slides In Vectashield, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/slides in vectashield/product/Vector Laboratories
Average 86 stars, based on 1 article reviews
slides in vectashield - by Bioz Stars, 2026-02
86/100 stars
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tris buffered saline  (Bio-Rad)
98
Bio-Rad tris buffered saline
Figure 5. (A) SIRT1, FoxO3a, p53 and <t>p21</t> protein levels in lung tissue samples, analyzed by western blot, with β‑actin as a loading control. (B) SIRT1 deacetylase activity in lung samples. All analyses were performed in triplicate. Values are presented as the mean + standard deviation. *P<0.05 vs. control group. SIRT1, sirtuin 1; FoxO3a, forkhead box O 3a; COPD, chronic obstructive pulmonary disease.
Tris Buffered Saline, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/tris buffered saline/product/Bio-Rad
Average 98 stars, based on 1 article reviews
tris buffered saline - by Bioz Stars, 2026-02
98/100 stars
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1:200 alexa fluor® 568 donkey anti-mouse  (Thermo Fisher)
90
Thermo Fisher 1:200 alexa fluor® 568 donkey anti-mouse
Figure 5. (A) SIRT1, FoxO3a, p53 and <t>p21</t> protein levels in lung tissue samples, analyzed by western blot, with β‑actin as a loading control. (B) SIRT1 deacetylase activity in lung samples. All analyses were performed in triplicate. Values are presented as the mean + standard deviation. *P<0.05 vs. control group. SIRT1, sirtuin 1; FoxO3a, forkhead box O 3a; COPD, chronic obstructive pulmonary disease.
1:200 Alexa Fluor® 568 Donkey Anti Mouse, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/1:200 alexa fluor® 568 donkey anti-mouse/product/Thermo Fisher
Average 90 stars, based on 1 article reviews
1:200 alexa fluor® 568 donkey anti-mouse - by Bioz Stars, 2026-02
90/100 stars
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supersignal west pico chemiluminescent substrate  (Thermo Fisher)
90
Thermo Fisher supersignal west pico chemiluminescent substrate
Figure 5. (A) SIRT1, FoxO3a, p53 and <t>p21</t> protein levels in lung tissue samples, analyzed by western blot, with β‑actin as a loading control. (B) SIRT1 deacetylase activity in lung samples. All analyses were performed in triplicate. Values are presented as the mean + standard deviation. *P<0.05 vs. control group. SIRT1, sirtuin 1; FoxO3a, forkhead box O 3a; COPD, chronic obstructive pulmonary disease.
Supersignal West Pico Chemiluminescent Substrate, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/supersignal west pico chemiluminescent substrate/product/Thermo Fisher
Average 90 stars, based on 1 article reviews
supersignal west pico chemiluminescent substrate - by Bioz Stars, 2026-02
90/100 stars
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polyclonal antibodies  (Santa Cruz Biotechnology)
99
Santa Cruz Biotechnology polyclonal antibodies
Figure 5. (A) SIRT1, FoxO3a, p53 and <t>p21</t> protein levels in lung tissue samples, analyzed by western blot, with β‑actin as a loading control. (B) SIRT1 deacetylase activity in lung samples. All analyses were performed in triplicate. Values are presented as the mean + standard deviation. *P<0.05 vs. control group. SIRT1, sirtuin 1; FoxO3a, forkhead box O 3a; COPD, chronic obstructive pulmonary disease.
Polyclonal Antibodies, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/polyclonal antibodies/product/Santa Cruz Biotechnology
Average 99 stars, based on 1 article reviews
polyclonal antibodies - by Bioz Stars, 2026-02
99/100 stars
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tmr nhs-ester  (Thermo Fisher)
90
Thermo Fisher tmr nhs-ester
Figure 5. (A) SIRT1, FoxO3a, p53 and <t>p21</t> protein levels in lung tissue samples, analyzed by western blot, with β‑actin as a loading control. (B) SIRT1 deacetylase activity in lung samples. All analyses were performed in triplicate. Values are presented as the mean + standard deviation. *P<0.05 vs. control group. SIRT1, sirtuin 1; FoxO3a, forkhead box O 3a; COPD, chronic obstructive pulmonary disease.
Tmr Nhs Ester, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/tmr nhs-ester/product/Thermo Fisher
Average 90 stars, based on 1 article reviews
tmr nhs-ester - by Bioz Stars, 2026-02
90/100 stars
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rabbit anti γ tubulin  (Bethyl)
93
Bethyl rabbit anti γ tubulin
Figure 5. (A) SIRT1, FoxO3a, p53 and <t>p21</t> protein levels in lung tissue samples, analyzed by western blot, with β‑actin as a loading control. (B) SIRT1 deacetylase activity in lung samples. All analyses were performed in triplicate. Values are presented as the mean + standard deviation. *P<0.05 vs. control group. SIRT1, sirtuin 1; FoxO3a, forkhead box O 3a; COPD, chronic obstructive pulmonary disease.
Rabbit Anti γ Tubulin, supplied by Bethyl, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit anti γ tubulin/product/Bethyl
Average 93 stars, based on 1 article reviews
rabbit anti γ tubulin - by Bioz Stars, 2026-02
93/100 stars
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secondary antibodies  (Thermo Fisher)
90
Thermo Fisher secondary antibodies
Figure 5. (A) SIRT1, FoxO3a, p53 and <t>p21</t> protein levels in lung tissue samples, analyzed by western blot, with β‑actin as a loading control. (B) SIRT1 deacetylase activity in lung samples. All analyses were performed in triplicate. Values are presented as the mean + standard deviation. *P<0.05 vs. control group. SIRT1, sirtuin 1; FoxO3a, forkhead box O 3a; COPD, chronic obstructive pulmonary disease.
Secondary Antibodies, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/secondary antibodies/product/Thermo Fisher
Average 90 stars, based on 1 article reviews
secondary antibodies - by Bioz Stars, 2026-02
90/100 stars
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Image Search Results


Figure 5. (A) SIRT1, FoxO3a, p53 and p21 protein levels in lung tissue samples, analyzed by western blot, with β‑actin as a loading control. (B) SIRT1 deacetylase activity in lung samples. All analyses were performed in triplicate. Values are presented as the mean + standard deviation. *P<0.05 vs. control group. SIRT1, sirtuin 1; FoxO3a, forkhead box O 3a; COPD, chronic obstructive pulmonary disease.

Journal: Molecular medicine reports

Article Title: LncRNA‑mediated SIRT1/FoxO3a and SIRT1/p53 signaling pathways regulate type II alveolar epithelial cell senescence in patients with chronic obstructive pulmonary disease.

doi: 10.3892/mmr.2017.6367

Figure Lengend Snippet: Figure 5. (A) SIRT1, FoxO3a, p53 and p21 protein levels in lung tissue samples, analyzed by western blot, with β‑actin as a loading control. (B) SIRT1 deacetylase activity in lung samples. All analyses were performed in triplicate. Values are presented as the mean + standard deviation. *P<0.05 vs. control group. SIRT1, sirtuin 1; FoxO3a, forkhead box O 3a; COPD, chronic obstructive pulmonary disease.

Article Snippet: The membranes were then incubated with primary antibodies against p53 (#9282; 1:1,000 in TBS-T; Cell Signaling Technology, Inc., Danvers, MA, USA), p21 (sc-397; 1:500 in TBS-T; Santa Cruz Biotechnology, Inc., Dallas, TX, USA), FoxO3a (#12829; 1:1,000 in TBS-T; Cell Signaling Technology, Inc.) SIRT1 (#2493; 1:1,000 in TBS-T; Cell Signaling Technology) and β-actin (ab8227; 1:1,000; Abcam, Cambridge, UK) overnight at 4 ̊C on an orbital shaker.

Techniques: Western Blot, Control, Histone Deacetylase Assay, Activity Assay, Standard Deviation

Figure 4. mRNA expression in lung tissues. SIRT1, FoxO3a, p53 and p21 mRNA expression levels were analyzed by reverse transcription‑quantitative polymerase chain reaction and quantified with the 2‑ΔΔCq method. All analyses were performed in triplicate. Values are presented as the mean + standard deviation. *P<0.05 vs. control group. SIRT1, sirtuin 1; FoxO3a, forkhead box O 3a; COPD, chronic obstructive pulmonary disease.

Journal: Molecular medicine reports

Article Title: LncRNA‑mediated SIRT1/FoxO3a and SIRT1/p53 signaling pathways regulate type II alveolar epithelial cell senescence in patients with chronic obstructive pulmonary disease.

doi: 10.3892/mmr.2017.6367

Figure Lengend Snippet: Figure 4. mRNA expression in lung tissues. SIRT1, FoxO3a, p53 and p21 mRNA expression levels were analyzed by reverse transcription‑quantitative polymerase chain reaction and quantified with the 2‑ΔΔCq method. All analyses were performed in triplicate. Values are presented as the mean + standard deviation. *P<0.05 vs. control group. SIRT1, sirtuin 1; FoxO3a, forkhead box O 3a; COPD, chronic obstructive pulmonary disease.

Article Snippet: The membranes were then incubated with primary antibodies against p53 (#9282; 1:1,000 in TBS-T; Cell Signaling Technology, Inc., Danvers, MA, USA), p21 (sc-397; 1:500 in TBS-T; Santa Cruz Biotechnology, Inc., Dallas, TX, USA), FoxO3a (#12829; 1:1,000 in TBS-T; Cell Signaling Technology, Inc.) SIRT1 (#2493; 1:1,000 in TBS-T; Cell Signaling Technology) and β-actin (ab8227; 1:1,000; Abcam, Cambridge, UK) overnight at 4 ̊C on an orbital shaker.

Techniques: Expressing, Polymerase Chain Reaction, Standard Deviation, Control